Crossed immunoelectrophoresis from sodium dodecyl sulfate- polyacrylamide gels after fixation and staining and without nonionic detergent intermediate layers.
نویسنده
چکیده
A modified method is described for crossed immunoelectrophoresis in which the first-dimension separation has been carried out by sodium dodecyl sulfateepolyacrylamide gel electrophoresis. The described method does not require nonionic detergents and is carried out after fixation and staining of the polyacrylamide gel. This permits more precise alignment of immunoprccipitatcs with polypeptide bands as well as allowing direct testing of an individual polypeptide band for reaction with antibody.
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ورودعنوان ژورنال:
- Analytical biochemistry
دوره 116 1 شماره
صفحات -
تاریخ انتشار 1981